Figure 5

Figure 5

UVB irradiation induces inflammation in zebrafish. Neutrophils reporter line Tg (mpo: GFP) was generated to detect inflammation. (A–E) Embryos aged 3 dpf without UVB irradiation at different time points. (F–J) Neutrophils number of caudal fin area over the intermediate cell mass from 3 h to 14 h post-irradiation was counted. (K) Number of neutrophils within 400 μm region (red box) was calculated. (F–G) Neutrophils did not shift to caudal fin before 9 h post-irradiation. (H) After 14 h post-irradiation, neutrophils started to migrate to caudal fin. (I,J) Neutrophils continuously migrated from 19 h after irradiation to 24 h. (K) Quantification of the neutrophils at caudal fin. Neutrophils migration to caudal fin begins from 14 h post UVB irradiation and continued to 24 h post UVB irradiation. (n = 10–20, t-test was used to determine the significance change, * p < 0.05, ** p < 0.01, *** p < 0.001).