ZFIN Image: Hansen et al., 2020, Figure 2

Image

Figure Caption

Figure 2—figure supplement 2. Subcelluar localization of nanobody-targeted optogenetic tools.

(A) HEK-TM cells expressing bPAC. HEK-TM cells stably expressing bPAC-mCherry (red) were fixed and labeled with DAPI (blue). Scale bar: 20 μm. (B) See A. for LAPD-mCherry. (C) HEK-TM cells expressing the cilia-targeted anti-mCherry mNphp3(201)-VHH_LaM-2-eGFP (green) nanobody. The box indicates the position of the magnified view shown at the bottom right. (D) HEK-TM cells expressing the anti-mCherry VHH_LaM-2-eGFP (green) nanobody. (E) Co-expression of the cilia-targeted anti-mCherry nanobody (LaM-2) (green) and bPAC-mCherry (red) in HEK-TM cells. The box indicates the position of the magnified view shown at the bottom: left: all channels as overlay; center: mCherry channel only; right: eGFP channel only. (F) Co-expression of the cilia-targeted anti-mCherry nanobody (LaM-2) (green) and LAPD-mCherry (red) in HEK-TM cells. The box indicates the position of the magnified view shown at the bottom: left: all channels as overlay; center: mCherry channel only; right: eGFP channel only.

Acknowledgments

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