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Fig 9

ID: ZDB-IMAGE-200711-31
Publication: Takamiya et al., 2020 - Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves
All Figures: Figures for Takamiya et al., 2020

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Figure Caption

Fig 9 Transplantation of a lens into <italic>pax6a/b</italic> double mutants partially restores AS morphology.

Intravenous injection of 2000 kDa dextran (green) and 3 kDa dextran (magenta) into wild type (A-B) and double homozygous pax6a-/-;pax6b-/- mutant embryos (C-D) at 5 dpf allows visualizing the anterior chamber (ac, magenta) with reference to ocular annular vessels (green) in en face view. The asterisk denotes diffusion of 3 kDa dextran dye through the uveoscleral pathway. (A) Wild type control anterior chamber with no surgical operation. (B) No anterior chamber was formed in wild type after lens removal at 24 hpf. (C-D) The anterior chamber was partially restored in pax6a/b double mutant eyes after transplanting a wild type lens at 24 hpf (C), while no anterior chamber was formed without operation (D). Orientations: dorsal (D), ventral (V), nasal (N) and temporal (T). Scale bar: 50 μm.

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