IMAGE

Fig. 4.

ID

ZDB-IMAGE-200703-29

Genes

myl7
nkx2-5

Publication

Sam et al., 2020 - Specificity, redundancy and dosage thresholds among gata4/5/6 genes during zebrafish cardiogenesis

All Figures

Figures for Sam et al., 2020

Image

Figure Caption

Fig. 4.

Genetic interactions between gata4 and gata5 impact progenitor migration and heart tube formation. ISH was performed using offspring from a gata4^+/−, gata5^+/− in-cross and probes targeting either myocardial marker myl7 or cardiac progenitor marker nkx2.5. (A) Progressive loss of gata4 and gata5 alleles results in a range of heart tube defects due to abnormal heart tube morphogenesis. Loss of a gata5 allele in gata4 mutants generates a thin heart tube, while loss of gata4 alleles partially rescues the gata5^−/− bifid phenotype. Representative scale bars in WT panels: 0.1 mm. (B) Quantification of nkx2.5 staining shown in A shows that loss of gata4 does not significantly affect cardiac specification. Error bars shown are s.e.m. *P<0.05, **P<0.01. (C) Schematic showing combinatorial loss of gata4 and gata5 disrupts proper fusion of the heart tube.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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