IMAGE

Fig. 5

ID
ZDB-IMAGE-200615-7
Source
Figures for Wang et al., 2020
Image
Figure Caption

Fig. 5 <italic>adamts3</italic> expression via motoneurons or mesenchymal cells can rescue venous sprouting and PL formation in <italic>adamts3</italic>; <italic>adamts14</italic> double mutants.

a Diagram depicting the heterochronic transplantation approach: Dextran-Alexa647 is injected into one-cell stage adamts14−/−; adamts3:Gal4FF; UAS:GFP embryos (donor embryos). Cells are transferred from donor embryos to adamts3; adamts14 double mutants at 6 hpf (host embryo) and the effects are assessed at 48 hpf. b, c In cases where transplanted cells contributed to adamts3-expressing motoneuron clusters (green arrowhead), venous ISVs (white arrowhead), and PL cells (white arrow) formed in double mutants. c Transplanted cells labeled by Dextran-Alexa647. d When transplants gave rise to adamts3+ cells at three locations, the HM, the ventral segment boundaries, and close to the DA and PCV, a local rescue of the formation of PL cells and intersegmental veins in adamts3; adamts14 double mutant embryos was visible. e Zoom-in of the boxed region in (d) with adamts3-expressing cells at the segment boundary highlighted by an arrow and transplanted cells at the HM marked by an arrowhead. f, g Transplantation of cells contributing to adamts3-expressing cells at the ventral segment boundaries (blue arrow) and to cells located close to the DA (magenta arrow) and the PCV (white arrow) also resulted in a locally restricted rescue of PL and vISV formation. g Zoom-in of the indicated region in f. h, i Cases, in which the transplants gave rise to adamts3+ cells at the HM (arrowhead in i) and the region around the PCV (arrow in i), but not to the cells along the ventral segment boundaries showed a rescue of PL cells and vISVs. j Overview about the results obtained from 23 rounds of cell transplantations. Each round contained 144 recipient embryos obtained from an adamts3+/−; adamts14−/− incross of which a quarter was expected to be double mutant (in total 828). Embryos showing a rescue of PL or vISV development were selected for imaging and genotyping. All embryos shown in (j) are adamts3; adamts14 double mutants. Scale bars: 50 µm. DA dorsal aorta, hpf hours post fertilization, PCV posterior cardinal vein, PL parachordal lymphangioblast, vISV venous intersegmental vessel.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.