ZFIN Image: Van Der Meulen et al., 2020, FIGURE 3

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Figure Caption

FIGURE 3

Periocular mesenchyme subpopulation distribution analysis. (A) 3D rendering of confocal stacks encompassing the AS in POM transgenic lines between 24 and 72 hpf. GFP+ cells are green, DNA was stained with DAPI (blue). DN, dorsal-nasal; DT, dorsal-temporal; VN, ventral-nasal; VT, ventral-temporal. Scale bar = 50 μm. (B) Distribution of quantified GFP+ cells within each AS quadrant (DN, DT, VN, and VT) at 24, 30, and 48 hpf. Distribution is represented as an average percentage of the total number of GFP+ in each quadrant of the AS for each transgenic line. Statistically significant difference (p < 0.05): * vs. DT, # vs. VN, $ vs. VT and ^ vs. DN. (C) Model of POM colonization at 24, 30, and 48 hpf. (D) Average AS cell population size for each transgenic line at 24–72 hpf. * indicates statistically significant (p < 0.05) change from previous time point.

Acknowledgments

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