Reduced blood flow changesklf2aandklf2bexpression during ventricle regeneration. (A–H) Whole-mount in situ hybridizations showing klf2a upregulation in ablated hearts (E–H) compared to control hearts (A–D) at 4–7 dpf/1–4 dpt. (I–P) Whole-mount in situ hybridizations showing klf2b upregulation in ablated hearts (M-P) compared to control hearts (I–L) at 4–7 dpf/1–4 dpt. (Q–V) Whole-mount in situ hybridizations indicated klf2a upregulation after ablation (Q, T) was blocked in Tricaine treated hearts (R, U) and BDM treated hearts (S, V) at 1 dpt. (W–B’) Whole-mount in situ hybridizations indicated klf2b upregulation after ablation (W, Z) was slightly enhanced in Tricaine treated hearts (X, A’) and BDM treated hearts (Y, B’) at 1 dpt. Scale bars, 50 µm. dpt, days post treatment. Dashed lines outline the hearts. Numbers indicate the ratio of representative staining observed
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