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Figure 4 - figure supplement 1 pgk1–<italic> / </italic>- mutants show normal onset of <italic>fgf3</italic> and <italic>fgf8a</italic>, but not <italic>pax5</italic>, in the otic vesicle.

(A–D) Expression of fgf3 and pax5 in wild-type and pgk1- / - mutant embryos at 18 hpf. fgf3 (A, B) is expressed in rhombomere 4 (r4) and pharyngeal endoderm (pe) and appears normal in pgk1- / - mutants, while otic expression of pax5 (C, D, arrows) is reduced in mutant embryos. (E–H) At 19 hpf, expression of fgf3 (E, F) and fgf8a (G, H) is reliably detected in the otic vesicle (arrows) and appears normal in pgk1- / - mutants. Images show dorsal views with anterior to the top.

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