Figure 4

Figure 4

Evaluation of in vivo RFM O activity on M. abscessus CIP 104536 R morphotype expressing mWasabi infection. Zebrafish (ZF) were infected with M. abscessus CIP 104536^T R expressing mWasabi, and treated with different antibiotics. The M. abscessus infected ZF were exposed to RIF, RFT, RFM O, RFB and CLR at a concentration of 25 μM and compared to untreated controls. Green fluorescent protein (GFP) dissemination in ZF was captured using fluorescent microscopy (A). The 5 dpi embryos at 25 µM of RIF, RFT, RFM O, RFB and CLR show significant reductions in infection burden (*** P < 0.0001; ns, not significant) (B). Survival of M. abscessus-infected embryos treated at 25 μM of rifamycin analogs and clarithromycin (C) in comparison with untreated infected embryos and non-treated control (n = 20, representative of three independent experiments). * P < 0.05, *** P < 0.001.