IMAGE

Fig 7

ID
ZDB-IMAGE-200429-7
Genes
Source
Figures for Arribat et al., 2020
Image
Figure Caption

Fig 7 Spastin influences transcription and location of HSP-related proteins to control ER/LD dynamics.

(A) Quantification of REEP1, Atlastin1, Spartin and Seipin transcript by qPCR in 8 months old zebrafish brain. n = 4 animals per group. (B) Quantification of REEP1, Atlastin1, Spartin and Seipin transcript by qPCR in 8 months old zebrafish skeletal muscle. n = 4 animals per group. (C-D) Confocal microscopy images of HeLa cells overexpressing human Seipin with Spastin M1 isoforms (wild-type and mutants) treated with oleic acid (OA) for 18h. Cherry-tagged Spastin appears in red, Seipin in green, LDs (Bodipy) in magenta and nucleus (Hoechst) in blue. (E) Percentage of cells exhibiting tubular ER along MTs. (F-G) Confocal microscopy images of HeLa cells overexpressing human REEP1 with Spastin M1 isoforms in basal conditions (F) or after 18h administration of OA (G). Cherry-tagged Spastin appears in red, REEP1 in green, LDs (Bodipy) in magenta and nucleus (Hoechst) in blue. Bars are mean ± SEM, #P = 0.08, *P < 0.05, **P < 0.01, ***P<0.001 (unpaired t‐test). See also S3 Fig and S4 Fig.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.