Figure 4

Figure 4

Distal Anchors in Sox2-Dependent Interactions Drive GFP Transgene Activity to Zebrafish Brain

Top: enhancer-dependent GFP-reporter (ZED): distal anchors (DA) from Sox2-dependent interactions are cloned upstream to a minimal promoter and GFP. Bottom: first and second left columns, GFP expression in transgenic embryos and bright-field images (F1 of stable lines, except for c-fos, transient transgenics). Third column: expression (in situ hybridization from http://zfin.org) of the endogenous zebrafish gene corresponding to the gene connected, in mouse, to the tested anchor. Fourth column: forebrain lacZ staining driven by transgenes carrying the human enhancers corresponding to the anchor (from https://enhancer.lbl.gov) (Visel et al., 2009).

See also Figure S6 and Table S6.