ZFIN Image: Scaletti et al., 2020, Figure 4.

Image

Figure Caption

Figure 4.

Activity with N6-methyl-dATP is unique to MTH1 within human NUDIX subfamily. Activities of human NUDIX enzyme (MTH1, NUDT15, NUDT17, and NUDT18) were assayed with data points in quadruplicate with 50 μm dATP or N6-methyl-dATP at 20 and 200 nm enzyme in MTH1 reaction buffer (pH 8.0). 0.2 units/ml of PPase was used to convert formed PP_i to P_i that was detected using malachite green reagent and measurement of absorbance at 630 nm. Graph shows mean ± S.D. from one experiment performed in triplicate.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.