ZFIN Image: S Alqahtani et al., 2020, Figure 4

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Figure Caption

Figure 4

HepG2 cell death after treatment with compounds CM1 and CM2. Cells were exposed to the IC₅₀ for 48 h, and cells undergoing either apoptosis or necrosis were assessed using co-staining with Annexin-V/FITC-PI dyes. (A) Representative scatter plots of PI (y-axis) vs. FITC-Annexin V (x-axis); live cells (A3) were negative for both dyes, (A4) early apoptotic cells were positive for only FITC -Annexin V, (A2) late apoptotic cells were stained with both dyes and(A1) Necrotic cells were stained with PI only. (B) Percentage of viable, early apoptotic, and late apoptotic + necrotic cells. Data are presented as the means ± SD of the triplicate experiments. ** p < 0.05 and *** p < 0.01 vs. control.

Acknowledgments

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