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Fig. 6 Development of the choriocapillaris in mice is dependent of VEGFR2 signaling. A: Confocal micrographs of the choriocapillaris (CCs) from embryonic day (E)12, 16, postnatal day (P)1 and adult BALB/c mice stained with anti-CD31 (endothelium shown in green). Yellow arrowheads indicate interstitial foci. B. Quantification of the number of interstitial foci in the experiment shown in A. n = 10?15 areas of interest from four embryos collected from two litters, ***:p < 0.001, **:p < 0.005. A: Adult. C. Confocal micrographs of the choriocapillaris from E16 BALB/c mice treated with antiVEGFR2 neutralizing antibody or control IgG antibody for 3 days before staining with anti-CD31 antibody and visualization on the right side. D: Quantification of the number of interstitial foci in the experiment shown in C. n = 10?15 areas of interest from four embryos collected from two litters, ***:p < 0.001. antiV: Anti-VEGFR2-treated. E: Quantification of the number of sprouts in the experiment shown in C. n = 10?15 areas of interest from four embryos collected from two litters, ***:p < 0.001.
Reprinted from Developmental Biology, 457(2), Ali, Z., Cui, D., Yang, Y., Tracey-White, D., Vazquez-Rodriguez, G., Moosajee, M., Ju, R., Li, X., Cao, Y., Jensen, L.D., Synchronized tissue-scale vasculogenesis and ubiquitous lateral sprouting underlie the unique architecture of the choriocapillaris, 206-214, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.