Figure 4

Figure 4

zCry mRNA levels under blue light/dark and dark conditions. Zebrafish were entrained to white light/dark cycles (14L:10D) and irradiated with blue (λ_max = 462 nm; λ_1/2 = 453 nm and 573 nm; blue circles) light or kept in darkness (black squares) at 28 °C for 14 h from ZT0 (Supplementary Fig. S1). Light intensities were set at 5.586 × 10¹⁴ photons s⁻¹ m⁻² that corresponds to 240 μW cm⁻². Tissues were collected from three (brain samples at ZT0 and ZT19, and eye samples at ZT7 and ZT19) or four (other samples) zebrafish at ZT0, ZT1, ZT3, ZT7, ZT14, ZT15, ZT19 or ZT0 (ZT24) (see Methods for details). Each mRNA level was estimated as a relative value to the geometric mean of mRNA levels of zβ-actin, zGapdh and zEf1α (arithmetic means of their CT values were used in the 2^−ΔΔCT method because they were stably expressed in the brain and eye). Data were analyzed by two-way ANOVA (Supplementary Table S33) and Tukey-Kramer post-hoc tests (Supplementary Tables S34–S57). Error bars represent ± SE.