Figure 5

Figure 5

Zebrafish injected with tick saliva and fed with red meat develop hemorrhagic anaphylactic-type reactions and desensitization (Experiment 1). (A) In the zebrafish model, 33% of animals treated with tick saliva on day 1 developed hemorrhagic anaphylactic-type allergic reactions 3–5 hpt and died. Furthermore, 100% of fish fed with dog food, but none of the fish that continued feeding on fish feed at day 2, developed allergy to tick saliva injected on day 3. Once recovered from anaphylactic-type reactions, all fish were desensitized and became tolerant to tick saliva injected on day 8. (B) Signs of hemorrhagic anaphylactic-type reactions in zebrafish No. 26-1, 26-2, and 26-3 injected with tick saliva and dying at day 2 before food change. After food change, only fish No. 14-7, 14-8, and 14-9 feeding on dog food developed anaphylactic-type reactions. Asterisks in red connect representative fish images in (A) with results in (B).