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Fig. 2-S1
EEC activity assay.
( A) Experimental design of EEC activity assay using Tg( neurod1:Gcamp6f) zebrafish. ( B) Representative images of EEC calcium fluorescence analysis using FIJI template matching and background subtraction in Tg( neurod1:Gcamp6f) zebrafish stimulated with palmitate. ( C) Relative fluorescence intensity in the proximal intestine in a series of video images from zebrafish in B. ( D) Spatial-temporal resolution of the EEC response to palmitate, glucose and cysteine stimulation. ( E) Representative images of the EEC nutrient response in a regional specific manner. Palmitate and glucose primarily activated EECs in the proximal intestine where most lipid and nutrient absorption occurs. In contrast, cysteine activated EECs in the mid-intestine (segment 2) where proteins and other macromolecules are digested andabsorbed by specialized intestinal epithelial cells in this region ( Nakamura et al., 2004; Wang et al., 2010; Park et al., 2019).