Fig. 6-S2
Treatment of Thapsigargin inhibited EEC response to nutrient stimulation.
( A–B) Representative heatmap image of Tg(neurod1:Gcamp6f) zebrafish in control ( A) or 2 hr post Thapsigargin treated conditions. Yellow lines in A and B indicate the proximal intestine area that used to quantify the fluoresence intensity in C. ( C) Quantification of basal fluoresence in the proximal intestine in control and Thapsigargin treatment zebrafish. ( D) Representative EECs’ temporal response to glucose stimulation in control and 2 hr post Thapsigargin treated zebrafish. ( E) Representative EECs’ temporal response to palmitate stimulation in control and 2 hr post Thapsigargin treated zebrafish.