Effects of ZnO-NPs on the activation of mTOR and p70S6K in human GSCC cells. (a) Quiescent Ca9-22 cells were treated with or without culture medium (10% fetal bovine serum (FBS)) in the absence (CTL) or presence of ZnO-NPs (20 µg/mL) or rapamycin (10 µM). (b) Quiescent OECM-1 cells were treated with ZnO-NPs (50 µg/mL). Then, cells were harvested and lysed for the detection of p-mTOR and p-p70S6K by Western blot analysis. Image-Pro Plus processing software quantified the relative level of phosphorylated protein. Data represent the mean ± SEM of five independent experiments. * p < 0.05 compared with the control group.
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