The assessment of the influence of gender on learning and memory in the T-maze test. (A) Comparison of the latency (s) for male (blue) and female (red) fish to swim into the punishment chamber for each training session. (B) Comparison of the time spent in punish arm (s) for male and female fish at different time points before and after training. (C) Comparison of the freezing time (s) for male and female fish at different time points before and after training. (D) Comparison of the total number of electric shock between male and female fish. (E) Comparison of the memory retention latency (s) for male and female fish at different time points after training. The data are expressed as means ± SEM. The data were analyzed by two-way ANOVA and followed with the Tukey–HSD test. Different letters (a, b, c) on the error bars represent significant differences (p < 0.05, n = 18).
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