Figure 1

Figure 1

Genetic organization of the σ^VreI regulon. Transcriptional organization of the vreAIR locus (black) and the downstream σ^VreI-regulated genes (colored). Block arrows represent the different genes, their relative sizes, and their transcriptional orientation, with the name of the gene or the PA number (http://www.pseudomonas.com/) indicated below the arrow. The promoters and regulatory boxes identified within this locus are indicated^19,20,62. Numbers indicate the fold-change in the expression of the gene in cells overproducing σ^VreI as determined earlier by microarray²¹. The hxc genes (dark green) encode a type II secretion system involved in the secretion of the low molecular weight alkaline phosphatase LapA (light green)⁶². pdtA and pdtB (blue) encode a functional two-partner secretion (TPS) system involved in P. aeruginosa virulence in the C. elegans model⁶³. phdA (yellow) encodes a homologue of the prevent-host-death (Phd) protein family and is required for biofilm formation and eDNA release⁶⁴. exbB2-exbD2-tonB4 genes (orange) encode a still uncharacterized putative TonB system. The function of the PA0696-PA0700 gene products (purple) is still unknown. PA0701 (dark grey) encodes a putative LysR-like transcriptional regulator and PA0701a (light grey), which is not annotated in the PAO1 genome but it is in the P. aeruginosa PA14 genome, encodes a putative AraC-like transcriptional regulator.