Figure 3

Figure 3

Wound healing and blastema formation, triggering the regeneration process, are not affected by LDIR after amputation in zebrafish. The caudal fin of adult Fli1:EGFP zebrafish was amputated at mid-fin level and immediately exposed or not to 0.5 Gy for 3 consecutive days. (A) Caudal fin regeneration was followed over time, and regenerated area was quantified (in pixels) using ImageJ software. Data are represented as mean ± SEM and two-way with repeated measures ANOVA test was used to determine differences between experimental groups; ns, non-significant. (B) The mRNA expression levels of mmp9 (wound-healing marker) and msxb (blastema formation marker) were determined by qRT-PCR and normalized to elongation factor 1. Data represent the relative mRNA expression in irradiated tissue compared to non-irradiated ones. Data are represented as mean ± SEM of 12 independent zebrafish and differences between groups was assessed by independent samples T-test; ns, non-significant.