Figure 6

Figure 6

Neutrophil recruitment to sites of infection and injury is not altered in cxcr3.3 mutants. A total of 100 CFU of Mm‐mCherry were injected in the hb ventricle of 2 day‐old WT, cxcr3.2, and cxcr3.3 mutant larvae to assess neutrophil (mpx: eGFP) recruitment to the infection site at 3 hpi. The number of cells that infiltrated the cavity was lower in cxcr3.2 mutants but remained unchanged in WT and cxcr3.3 mutants (A and B). The tail fin of WT larvae and cxcr3.2 and cxcr3.3 mutants was amputated and neutrophil recruitment was assessed at 4 h postamputation. There were fewer recruited neutrophils in the cxcr3.2 mutants, whereas there was no difference between cxcr3.3 mutants and WT. The PBS‐injected control group PBS combines WT, cxcr3.2, and cxcr3.3 mutants and shows no cell recruitment at 3 hpi. In all cases, statistical analyses were done with pooled data of 3 independent replicates (20–30 larvae per group each). A Kruskal‐Wallis test was used to assess significance (ns P > 0.05, ^***P ≤ 0.001, ^****P ≤ 0.0001) and data are shown as mean ± sem