P-HAP protected zebrafish larvae from oxidative stress induced by alcohol consumption. (A) Fluorescence micrographs of ROS generation in the control zebrafish larvae and the zebrafish larvae treated with 350 mM ethanol or 50 μM p-HAP. Figures are magnified as 40×. (B) Quantification of the amounts and distribution of superoxide radicals according to fluorescence intensity. (C) Fluorescence micrographs of glutathione radical generation in zebrafish larvae. Figures are magnified as 40×. (D) Quantification of the amounts and distribution of glutathione anions according to fluorescence intensity. Data are presented as the mean ± SD. ##P < 0.01, ###P < 0.001 vs control group. ***P < 0.001 vs model group.
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