Innate immunity can be trained to control S. sonnei in vivo.
A. Model for S. sonnei reinfection. 2 dpf embryos were primed with PBS or a sublethal dose of S. sonnei. At 48 hours post primary infection (hp1i), larvae where challenged with a lethal dose of S. sonnei and monitored by survival assay for 72 hours post-secondary infection (hp2i). B,C. Innate immune training to S. sonnei is dependent on O-antigen. Survival curves (B) and Log10-transformed CFU counts (C) of 4 dpf larvae infected in the HBV with lethal dose (~8000 CFU) of mCherry-WT S. sonnei. 48 hours prior to infection with lethal dose, embryos were primed by delivering PBS (grey), or a sublethal dose (~80 CFU) of GFP-ΔO-Ag (blue) or GFP-WT (red) S. sonnei. Experiments are cumulative of 4 (B) or 3 (C) biological replicates. In C, full symbols represent live larvae and empty symbols represent larvae that at the plating timepoint had died within the last 16 hours. Statistics: Log-rank (Mantel-Cox) test (B); one-way ANOVA with Sidak’s correction on Log10-transformed data (C); ns, non-significant; *p<0.0332; ***p<0.0002; ****p<0.0001.
ZFIN wishes to thank the journal for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Your Input Welcome
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if
additional information is required.
Oops. Something went wrong. Please try again later.