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Fig. S2
Administration of 25-HC at stages post fertilization does not induce cell death: Acridine Orange staining on larvae treated with 25-HC at 1 dpf (A) and 2 dpf (B) showed no cell death in the brain and spinal cord. The graphs represent quantification of the net fluorescence upon Acridine orange staining. Each trial has a n of 7 for control and treated, repeated over 3 trials. A,P: Anterior and posterior side of the embryos respectively.
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