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Fig. S1
25-HC administration had no effect on Mauthner cells but caused cell death: (A) An immunostaining of 3A10, specific for Mauthner cells, showed no difference in the development of the cells. Acridine orange staining at 2 dpf in treated and un-treated larvae showed an increased cell death in the brain (white arrow heads) (B) and spinal cord (white arrow heads)(C) in a dose dependent manner. Each trial has a n of 7 for control and treated, repeated over 3 trials. A,P: Anterior and posterior side of the embryos respectively.
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