Fig. 3
Effects of 25-HC on the neuromuscular system in zebrafish larvae: (A) The spinal cord of control and treated larvae at 2 dpf, stained for acetylated α-tubulin, showed a significant reduction in the axon length of the motor neurons (white arrow heads) at 40 μM. (B) Staining for α-bungarotoxin in the muscles of treated and control larvae show no significant difference in the number of postsynaptic puncta. (C) Phalloidin staining of muscles in 25-HC-treated fish and controls at 2 dpf. All comparisons are performed with control, each trial has a n of 7 for control and treated over 4 trials. ** represents a significance of p < 0.01, ns: not significant. Significance was determined using one-way ANOVA and all data are represented as Mean ± SEM.