Fig. 2
25-HC affects early motility in zebrafish larvae: (A) Representation of the number of early spontaneous coiling of the larvae at 20 hpf (hours post fertilization) treated with 25-HC. There is a decrease in a dose dependent manner. (B) The distance moved by 5 dpf larvae upon treatment with 25-HC were assessed using the Ethiovision software, show a highly significant reduction in a dose dependent manner. (C) The figure represents motion snapshots of touch response in two representative trials (1,2) (N = 10) over 120 miliseconds (ms). At 40 μM, the larvae also have very little to no escape response to touch at 2 dpf. All comparisons are performed with control, each trial has a N of 7 for control and treated over 4 trials. * represents a significance of p < 0.05, **** represents a significance of p < 0.01. Significance was determined using one-way ANOVA and all data are represented as Mean ± SEM.