Fig. 1
25 Hydroxycholesterol affects development and is lethal upon chronic exposure at higher doses: The percentage of embryos surviving decreases (A) and hatching among the surviving embryos is delayed (B) with increase in the concentration of 25−HC. (C) morphological defects in development of the embryos at 2 dpf (days post fertilization), viewed and imaged using a bright field microscope. Defects get severe with increasing concentration of 25-HC. (D) The percentage of dead embryos and larvae with abnormal phenotypes (with heart edema and curved tails) increases in a dose dependent manner upon chronic exposure.