Fig. 2

Fig. 2

a Representative western blot analysis figure showing PRL-3 protein expression in Jurkat T-ALL cells 4d post-infection with lentivirus carrying shRNA. Numbers represent relative expression of PRL-3 protein, normalized to total protein loaded and compared to scrambled (SCR) control. b Cells infected with SCR or PRL-3 knock-down shRNA were cultured in media 4 days post-infection with 5 μg/ml puromycin for 72 additional hours. Cell growth was determined by Cell Titer-Glo assay and normalized to the readout of day 0, and shows no difference between groups. Data shown are the average of three independent experiments, done in triplicate, NS = not significant. c Knock-down of PRL-3 in Jurkat cell line reduced migration towards a serum stimulus more than 50%. Migration was normalized to the cells infected by SCR shRNA, p < 0.05 compared to SCR control, *p < 0.05. d Schematic diagram of the xenotransplantation assay. e Representative flow cytometry analysis of submandibular blood sample after human CD45 staining. f Quantification of human CD45 staining of blood from mice at week 4,6, and 8 after transplantation. Each dot represents one mouse, the horizontal line represents the mean value, and the standard deviation is shown, *p < 0.01 and **p < 0.001 compared to shRNA control xenografted mice.