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Figure 4
Bucillamine inhibited afatinib-mediated inhibition of EGFR autophosphorylation and downstream signaling. A431 cells expressing endogenous WT EGFR were pre-incubated with 0.01–1 μM afatinib in the presence or absence of 1 mM bucillamine. Then, 100 ng/mL of EGF was added to the culture medium. After 15 min incubation, cell lysates were prepared and analyzed by Western blotting with specific antibodies, as indicated. Shorter-exposure images are shown on the right. EGFR, epidermal growth factor receptor; WT, wild type.
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