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Figure 5—figure supplement 4.

ID: ZDB-IMAGE-200125-39
Publication: Arora et al., 2020 - Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish
All Figures: Figures for Arora et al., 2020

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Figure Caption

Figure 5—figure supplement 4. EC1-EC2 domain of E-cadherin is required to maintain Lgl2 levels in the epidermis.

Confocal images showing expression and localisation of GFP tagged E-cadherin-FL and ΔEC1-EC2-Ecad (green) (A1, B1, C1) and localisation of E-cadherin (magenta) (A2, A4, B2, B4, C2), Lgl2 (A3, A5, B3, B5) and aPKC (C3, C4). Lgl staining in the periderm (A3, B5) or basal epidermis (B3, A5) is shown as heatmap with calibration bar provided for reference (A6, B6). Arrow shows high levels of Lgl in the ΔEC1-EC2-Ecad expressing cells or juxtaposed cells (A3, A5, B3, and B5). Dotted lines indicate the position of the clone in the periderm (A1–A3 and C1-C4) or basal epidermis (B1–B3) and corresponding region in basal epidermis (A4–A5) or periderm (B4–B5), respectively. Scale bar represents 10 µm in (A5, B5, C4).

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