FIGURE 5

FIGURE 5

Slc7a14-deficient zebrafish morphants showed defective visual behaviors. (A,B) VMR testing in slc7a14-deficient zebrafish morphants. Larvae injected with slc7a14-MO (6.0 ng) showed a weaker ON response and a significantly attenuated OFF response compared to control larvae. N = 12 in each group. (C,D) Quantification of the VMR. N = 12 in each group. (C) Larvae injected with slc7a14 MO (6.0 ng) showed a reduced ON response compared with control larvae. (D) Compared with control larvae, larvae injected with 4.0 ng MO and 6.0 ng slc7a14-MOs exhibited markedly reduced OFF responses. (E,F) OKR testing demonstrated significant reductions in eye movement in the slc7a14-deficient zebrafish morphants compared to the control zebrafish. N = 10 in each group. VMR testing and OKR testing were repeated three times, respectively. Data was analyzed using One-way ANOVA followed by Games–Howell test, ^∗P ≤ 0.05, ^∗∗∗P < 0.001 significantly different from control 6.0 ng group.