Figure 3

Figure 3

MiR-17-5p knockdown suppressed the proliferation of CNE2. A: MiR-17-5p expression in 10 NPC tissues and 5 normal nasopharyngeal tissues by Real-time PCR analysis. B: Relative miR-17-5p expression in NPC cell lines and normal nasopharyngeal epithelial cell line. U6 was an endogenous control. (C-D): Immunofluorescence analysis and Real-time PCR analysis of miR-17-5p expression after CNE-2 cells transfected with plasmids. E: Proliferation of CNE-2 cells was examined using CCK-8 assay after transfected with miR-17-5p inhibition plasmid, miR-17 plasmid and negative control. F: Cell cycle analysis by flow cytometry in CNE-2 cells containing different levels of miR-17-5p. The data shown were representative of at least three independent experiments. * p < 0.05. ** p< 0.01.