PUBLICATION
Exosomal miR-17-5p promotes angiogenesis in nasopharyngeal carcinoma via targeting BAMBI
- Authors
- Duan, B., Shi, S., Yue, H., You, B., Shan, Y., Zhu, Z., Bao, L., You, Y.
- ID
- ZDB-PUB-191130-4
- Date
- 2019
- Source
- Journal of Cancer 10: 6681-6692 (Journal)
- Registered Authors
- Keywords
- BAMBI, angiogenesis, exosome, miR-17-5p, nasopharyngeal carcinoma
- MeSH Terms
- none
- PubMed
- 31777597 Full text @ J Cancer
Citation
Duan, B., Shi, S., Yue, H., You, B., Shan, Y., Zhu, Z., Bao, L., You, Y. (2019) Exosomal miR-17-5p promotes angiogenesis in nasopharyngeal carcinoma via targeting BAMBI. Journal of Cancer. 10:6681-6692.
Abstract
Objective: The purpose of our study is to investigate the role of miR-17-5p in angiogenesis of nasopharyngeal carcinoma and the crosstalk between HUVECs and CNE-2 via exosomes. Methods: Firstly, flow cytometry, cell viability assay, transwell assay, and tube formation were used to explore the role of miR-17-5p in angiogenesis. Then zebrafish model was used to confirm effects of miR-17-5p on angiogenesis. qRT-PCR analysis and Immunofluorescence assay were used to explore the expression of miR-17-5p in NPC tissues and cells compared to the normal control. Besides, in vitro assays were used to analyze the biological functions of miR-17-5p in NPC. What's more, in vitro and in vivo assays were used to detect the function of exosomal miR-17-5p in angiogenesis. Finally, luciferase reporter assay and western bolt were used to determine the relationship between miR-17-5p and BAMBI. Results: We observed that high expression of miR-17-5p promoted angiogenesis in NPC. Also, high expression of miR-17-5p promoted the NPC cells proliferation and migration. To know whether there's any communication between HUVECs and NPC cells, exosomes derived from CNE-2 cells were collected. Further results showed that exosomal miR-17-5p secreted from NPC promoted the angiogenesis. What's more, in vitro assays revealed that miR-17-5p targets BAMBI and regulates AKT/VEGF-A signaling. Conclusions: Our study showed that exosomal miR-17-5p derived from NPC cells promotes angiogenesis via targeting BAMBI and regulates AKT/VEGF-A signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping