Fig 2

Fig 2

Morphological observation of liver after the liver green fluorescence transgene zebrafish T3 (lfabp:EGFP) is exposed for 72h at blank (a and a’) and at ethanol concentrations of 0.6 mM(b and b’), 0.8 mM(c and c’), 1.0 mM(d and d’), 1.2 mM(e and e’) and 1.5 mM(f and f’). (A) Test of fluorescence intensity of zebrafish larvae liver in figure 2A with Image-Pro Plus 6.0 software. At least 3 zebrafish were tested in each group of concentration. Baseline adjusted values (mean±SEM) are presented. *P<0.05,**P<0.01 versus control. (B) Pathological observation of zebrafish liver tissue treated in blank (a) and various ethanol concentrations of 0.6 mM (b), 0.8 mM (c), 1.0 mM (d), 1.2 mM (e) and 1.5 mM (f), respectively. The liver part was marked with a red circle. Liver cell fatty degeneration was pointed out by the black arrow. Microvesicular lipid droplets in the liver cell were pointed out by the blue arrow.