Figure 8

Figure 8

Propagation of Ca²⁺ transients along the trunk and tail in embryos expressing Twitch-4 and GA. (A) Transmitted light and emission ratio at rest and during a Ca²⁺ transient in a Twitch-4 expressing embryo at 28 hpf. The calibration square shows 100 µm, hue codes for emission ratio, and intensity codes for fluorescence. Fluorescence images were acquired at 33 Hz. ROIs were drawn over somites in groups of 3 (colored traces). The graph shows the time course of a Ca²⁺ transient in the indicated color-coded ROIs. (B) Snapshots of a Ca²⁺ transient in a GA-expressing embryo with luminescence overlaid on transmitted light images. ROIs were selected on the indicated somites in the left panel in groups of three (colored traces). Images were acquired at 11.9 Hz. The scale bar represents 100 µm. The color scale indicates RLU. The graph shows the time course of luminescence during two contractions on the indicated somites. The snapshots on the left correspond to the first contraction (*).