ZFIN Image: Harris et al., 2019, Figure 4

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Figure 4

DrFKBP52 mutants potentiate androgen receptor activity. (A) Hormone-dependent β-galactosidase reporter gene activity was measured in yeast strains expressing wild type AR in the presence of 3 nM DHT. (B) FKBP activities were similarly determined in transfected 52KO MEF cells expressing wild-type AR and a hormone-dependent luciferase reporter. Protein expression levels were monitored by Western blot for the introduced FKBPs, AR, and endogenous GAPDH as a loading control. In all cases (A and B), androgen receptor activity in cells expressing DrFKBP52-A111V/T157R was significantly higher (p ≤ 0.0001) as compared with cells expressing Vector and DrFKBP52. There was no significant difference in androgen receptor activity in cells expressing human FKBP52 and DrFKBP52-A111V/T157R.

Acknowledgments

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