Figure 1

Figure 1

DrFKBP52 contains all known critical domains and residues for function but does not potentiate receptor activity. (A) Amino acid sequence alignment for human FKBP52 (HsFKBP52, Genbank accession number NP_002005.1) and Danio rerio FKBP52 (DrFKBP52, Genbank accession number NP_958877) indicates that DrFKBP52 has 61% similarity to HsFKBP52. The FK1, FK2, and TPR domains are indicated in gray. Previously characterized domains critical for function including the proline-rich loop (a), FK linker and CKII phosphorylation site (b), and conserved C-terminal tail motif important for Hsp90 binding (c) are indicated. (B) Dihydrotestosterone-induced AR-mediated β-galactosidase reporter assays were performed in yeast in the presence or absence of the indicated expression vectors for DrFKBP52, HsFKBP52, and HsFKBP51. In all cases, androgen receptor signaling in cells expressing HsFKBP52 was significantly higher (p ≤ 0.0001) as compared with cells expressing Vector, HsFKBP51, and DrFKBP52.