Figure 3

Figure 3

Effect of OJe on visceral and subcutaneous adipocyte size. Histological features of abdominal adipose tissue in sagittal sections (hematoxylin and eosin staining) showing visceral (A–D,I) and subcutaneous (E–H,L) adipocyte size in the four experimental groups. OJe treatment significantly reduced both visceral (A–D,I) and subcutaneous (E–H,L) average adipocyte size in both NF and OF fish. Photos (A–H) are representative of that (three per section) taken in 10 fish per group. Bar graphs show the results of the morphometric analysis of fat performed in both visceral (I) and subcutaneous (L) adipocyte average size in the four experimental groups (NF, NF + OJe, OF, and OF + OJe). Scale bars correspond to 20 µm. Results from the morphometric analysis in graphs (I) and (L) are expressed in µm² of adipocyte size and expressed as mean ± SD of 15 fish per each group. *** p < 0.001 vs. respective counterparts; ° p < 0.001 vs. NF.