ZFIN ID: ZDB-IMAGE-191230-1308
Figures for Roca et al., 2019

Figure Caption/Comments:

Figure 3

BAX Promotes Ca2+ Flow from the ER into the Mitochondrion

(A) Percentage of Rhod-2-positive macrophages in 1 dpi control and TNF-high larvae treated with Ru360. Horizontal bars, means; ∗∗p < 0.01; ∗∗∗p < 0.001 (one-way ANOVA with Tukey’s post-test). Representative of 2 independent experiments.

(B) Cording in 5 dpi TNF-high and control larvae treated with Ru360. p < 0.05 (Fisher’s exact test). Representative of 5 independent experiments.

(C) Percentage of Rhod-2-positive macrophages in 1 dpi control and TNF-high larvae that are WT or BAX mutant. Horizontal bars, means; ∗∗∗p < 0.001 (one-way ANOVA with Tukey’s post-test). Representative of 2 independent experiments.

(D) Percentage of Rhod-2-positive macrophages in 1 dpi control and TNF-high larvae treated with desipramine. Horizontal bars, means; p < 0.05 (one-way ANOVA with Tukey’s post-test).

(E) Percentage of Rhod-2-positive macrophages in 1 dpi control and TNF-high larvae that are WT, cathepsin D, or BID morphant. Horizontal bars, means; ∗∗∗p < 0.001 (one-way ANOVA with Tukey’s post-test). Representative of 2 independent experiments.

(F) Percentage of Rhod-2-positive macrophages in 1 dpi control and TNF-high larvae that are WT, BAX mutant, or BAX mutant expressing ΔBH3-BAX, MOM- or ER-targeted ΔBH3-BAX. Horizontal bars, means; ∗∗∗p < 0.001 (one-way ANOVA with Tukey’s post-test). Representative of 2 independent experiments.

(G) Cording in 5 dpi TNF-high or control larvae that are WT, BAX mutant, BAX mutant expressing ΔBH3-BAX, or BAX mutant expressing ER-targeted ΔBH3-BAX. ∗∗p < 0.01 (Fisher’s exact test). Representative of 2 independent experiments.

(H) Percentage of Rhod-2-positive macrophages in 1 dpi control and TNF-high larvae treated with thapsigargin. Horizontal bars, means; ∗∗∗p < 0.001 (one-way ANOVA with Tukey’s post-test).

(I) Cording in 5 dpi TNF-high or control larvae treated with thapsigargin. p < 0.05; ∗∗p < 0.01 (Fisher’s exact test). Representative of 3 independent experiments.

(J) Percentage of Rhod-2-positive macrophages in 1 dpi control and TNF-high larvae that are WT or overexpressing TMBIM3 or TMBIM6. Horizontal bars, means; ∗∗∗p < 0.001 (one-way ANOVA with Tukey’s post-test). Representative of 2 independent experiments.

(K) Cording in 5 dpi TNF-high or control larvae that are WT or overexpressing TMBIM3 or TMBIM6. p < 0.05; ∗∗p < 0.01 (Fisher’s exact test). Representative of 2 independent experiments.

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