Alcian blue staining of the seven pharyngeal arches of zebrafish embryos from 4 to 6 dpf. (a,a′) Control embryo formed five ceratobranchial (CB) bones at 4 dpf. (b,b′) SMF-exposed embryo developed only three CB bones. (c,c′,d,d′) Pharyngeal arches of 5 dpf control (c,c′) and SMF-exposed (d,d′) embryos displayed no significant difference. (e,e′,f,f′) Pharyngeal arches of 6 dpf control (e,e′) and SMF-exposed (f,f′) embryos displayed no significant difference. The seven pharyngeal arches are Meckel's cartilage (M), ceratohyal (CH) bone and the five pairs of ceratobranchial (CB1–5) bones as illustrated in e′. (a–f) use the same scale bar, 1 mm. (a′–f′) are the magnifications, respectively, and use the same scale bar, 0.5 mm.
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