FIGURE 5

FIGURE 5

Behavior of vldlr^–/– zebrafish. (A,B) Social preference test. (A) Both genotypes prefer to spend time near a group of unfamiliar fish [1st strangers; p < 0.0001 for both genotypes; two-way ANOVA followed by Sidak’s post hoc, genotype factor: F(1,24) = 0.0039, p = 0.95, stranger factor: F(1,24) = 120.1, p < 0.0001, interaction genotype × stranger: F(1,24) = 0.4912, p = 0.49]. (B) WT and vldlr^–/– spend equal time near both groups when a second unfamiliar group is added [p = 0.58 for WT and p = 0.73 for vldlr^–/–; two-way ANOVA followed by Sidak’s post hoc, genotype factor: F(1,24) = 1.353, p = 0.26, stranger factor: F(1,24) = 0.005, p = 0.94, interaction genotype × stranger: F(1,24) = 0.02779, p = 0.87. n = 8 wild-type and n = 7 vldlr^–/–]. (C–E)vldlr^–/– behavior in the open field test. (C)vldlr^–/– spent the same time at side of the tank [t-test (Welch): t_(1.581) = 9.068, p = 0.15] compared to WT but more (D) time in center of the tank [t-test (Welch): t_(2.595) = 9.47, p = 0.03]. (E) Locomotion [t-test (Welch): t_(0.3779) = 12.84, p = 0.71. n = 8 wild-type and n = 7 vldlr^–/–]. (F)vldlr^–/– exhibit normal anxiety-like behavior. Time at the bottom of novel tank (Mann Whitney test: U = 16, p = 0.32), (G) locomotion in a novel tank [t-test (Welch): t_(0.1803) = 10.92, p = 0.86. n = 7 wild-type and n = 7 vldlr^–/–]. (H) No difference in aggression levels between WT and vldlr^–/– [t-test (Welch): t_(1.87) = 11.41, p = 0.09. n = 8 WT and n = 7 vldlr^–/–]. ^∗p < 0.05, ^****p < 0.0001.