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Fig. 4
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Figure Caption
mab21l2 −/− mutants possess colobomas of varying severity, and retain basement membrane markers in the choroid fissure. A,D: Whole‐mount images of wild‐type (A) and mab21l2 −/− mutant (D) embryos highlighting colobomas. Compared with wild‐type, mab21l2 −/− embryos show colobomas of varying severities at 5 dpf (D). B,C,E,F: Transverse (B,E) and sagittal (C,F) sections of wild‐type (B,C) and mab21l2−/− mutant (E,F) embryos at 5 dpf. Note the severe coloboma in proximal eye cup of the mab21l2 −/− mutant (E, arrow) compared with wild‐type (B). In sagittal section view, (C,F), the mab21l2 −/− retina (F) displays discontinuity of retinal lamina and failure of choroid fissure fusion (arrowhead) when compared with wild‐type (C). G‐J′: Laminin α1 localization in wild‐type (G,H′) and mab21l2 −/− (I,J′) eyes. Magenta = laminin, cyan = DAPI. Le = lens. Arrowhead marks the site of the choroid fissure. Note that wild‐type embryos (G,G′,H,H′) do not display laminin α1 at the site of the closed choroid fissure, while mab21l2 −/− mutants (I,I′,J,J′) retain laminin α1 localization at the open choroid fissure (arrowheads). Scale bars = 50 μm
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