Fig. S4

Fig. S4

foxc1b:EGFP is expressed in vascular smooth muscle cells on the ventral aorta at larval stages. Ventral images of zebrafish embryos at 9 and 12 dpf. A) At 9 dpf, foxc1b:EGFP positive cells (arrowheads) are located adjacent to the kdrl:mCherry expressing endothelial cells of the ventral aorta (n = 4 embryos). B) At 9 dpf, foxc1b:EGFP and acta2:mCherry are co-expressed in mural cells (arrows) with the exception of a few single-positive cells (arrowheads; n = 4 embryos). C) At 12 dpf, foxc1b:EGFP positive cells continue to associate with endothelial cells of the ventral aorta (n = 6 embryos). D) At 12 dpf, most mural cells co-express foxc1b:EGFP and acta2:mCherry, although there are some single positive cells (n = 6 embryos). Grey colour in model indicates endothelial patterns. Scale bars represent 50 µm.