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Fig. 1
foxc1b:EGFP expressing mesenchymal cells attach to vessels and take on a vSMC morphology. A) Images of the ventral head in 2 dpf zebrafish embryos show foxc1b:EGFP positive mesenchymal cells near the endothelium (kdrl:mCherry; n = 19 embryos). B) At 3 dpf, foxc1b:EGFP positive cells associate with the endothelium (n = 14 embryos). Arrowheads indicate mural cells associated with the endothelium. C) At 4 dpf, foxc1b:EGFP positive cells flatten and tightly associate with the endothelium (n = 11 embryos). Scale bars represent 50 μm.
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Reprinted from Developmental Biology, 453(1), Whitesell, T.R., Chrystal, P.W., Ryu, J.R., Munsie, N., Grosse, A., French, C.R., Workentine, M.L., Li, R., Zhu, L.J., Waskiewicz, A., Lehmann, O.J., Lawson, N.D., Childs, S.J., foxc1 is required for embryonic head vascular smooth muscle differentiation in zebrafish, 34-47, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.