Fig. 2

Fig. 2

Liver-specific induction of Kras^G12V results in larger livers in ankrd45 mutants. (A–B”) Fluorescent in situ hybridization results showing the expression of ankrd45 in the liver of 5 dpf wild-type zebrafish larvae. Sense probe of ankrd45 was used as a negative control (B–B”). (C) Statistical results showing the average size of livers in control larvae (EGFP-kras^G12V heterozygote transgene) or ankrd45 larvae (ankrd45 homozygous mutants carrying EGFP-kras^G12V heterozygote transgene) at different time points after doxycycline treatment as indicated. Each dot represents the size of one larva. The average size of the livers at 1 day post treatment (dpt) in the control larvae was set as 100%. The number of larvae examined is shown at the bottom. (D) Representative images showing EGFP-Kras^G12V expression in the livers of control and ankrd45 mutants at different time points after doxycycline treatment. (E,F) Hematoxylin and eosin staining results showing the multiple cyst-like regions (asterisks) in the livers of ankrd45mutants after treatment. (G,H) Confocal images showing the apoptotic cells stained by the TUNEL assay. (I) Dotted graph showing the number of apoptotic cells in the livers of control and ankrd45 mutants 3 days after treatment. (J,K) Confocal images showing BrdU positive cells in the livers of control and mutant larvae as indicated. (L) Dotted graph showing the number of BrdU positive cells in the livers of control and ankrd45 mutants 3 days after treatment. Scale bars: 25 μm in panels A–B”, 200 μm in panel D, and 25 μm in panels G–K. ** p < 0.01, *** p < 0.001.