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Fig. S3
Localization of ANKRD45 in different cell lines
(A-D) Confocal images showing the localization of ANKRD45 in the midbody ring during cytokinesis of different cells as indicated. (E-F) Pre-incubation with synthesized ANKRD45 protein blocked the staining of ANKRD45 antibody in the midbody ring of U2OS cells. (G) Western blotting results showing the expression of ANKRD45 and cleaved PARP in control or four ANKRD45 siRNAs treated HeLa Cells. (H) Bar graph showing the relative expression level of ANKRD45 in control or siRNA treated HeLa cells. Scale bar: 5 μm.
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