Fig. 10

Fig. 10

Endothelial‐specific excision of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488‐rescuing transgene causes no apparent further impairment to tagln‐expressing perivascular mural cells in ift54 ^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 fish. A: In situ hybridization for tagln expression on transverse posterior trunk frozen sections of 13‐dpf wt (wt) and sibling ift54 ^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 ^/+ (ift54−/−;ubi:ift54) and ift54 ^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 ^/+; Tg(kdrl:cre)s898^/+ (ift54−/−;ubi:ift54;kdrl:cre) fish. The red line in the cartoon illustrates where sections were taken. Tagln expression is detected at the site of arteries such as the dorsal aorta (arrow). The Tg(kdrl:cre)s898 ^/+ caused no apparent further impairment to perivascular mural cells in the fish with partial transgenic rescue of ift54 ^tp49. Images representative of four animals analyzed for each genotype. B: In situ hybridization for tagln expression on transverse caudal fin frozen sections of adult wt (wt) and sibling ift54 ^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 ^/+ (ift54‐;ubi:ift54) and ift54 ^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 ^/+; Tg(kdrl:cre)s898 ^/+ (ift54‐;ubi:ift54;kdrl:cre) fish. The red line in the cartoon illustrates where sections were taken. The Tg(kdrl:cre)s898 ^/+ caused no apparent further impairment to tagln expression in the fish with partial transgenic rescue of ift54 ^tp49. Images representative of four animals analyzed for each genotype. WT, wild‐type